SelectedWorks - Lisa R. Warner

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Selected Works of Lisa R. Warner

Assistant Research Professor, Biomolecular Research Center

Joining Boise State University in October 2015, Dr. Lisa Warner is currently an Assistant Research Professor with the Biomolecular Research Center on campus. She earned her Ph.D. in Biochemistry from the University of Colorado, Boulder in 2011 and a B.S. in Chemistry from Boise State in 2002. Dr. Warner’s previous professional experience includes being a Postdoctoral Researcher for the National Renewable Resource Laboratory in Golden, Colorado, and an EMBO Postdoctoral Fellow for the Helmholz Zentrum München/Techniche Universität München in Munich, Germany. Her research interests include Biomolecular NMR, protein purification, and protein-protein interaction to name a few. Dr. Warner is currently an associate member in Structural Biology for the Faculty of 1000.

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About Lisa R. Warner

Joining Boise State University in October 2015, Dr. Lisa Warner is currently an Assistant Research Professor with the Biomolecular Research Center on campus. She earned her Ph.D. in Biochemistry from the University of Colorado, Boulder in 2011 and a B.S. in Chemistry from Boise State in 2002. Dr. Warner’s previous professional experience includes being a Postdoctoral Researcher for the National Renewable Resource Laboratory in Golden, Colorado, and an EMBO Postdoctoral Fellow for the Helmholz Zentrum München/Techniche Universität München in Munich, Germany. Her research interests include Biomolecular NMR, protein purification, and protein-protein interaction to name a few. Dr. Warner is currently an associate member in Structural Biology for the Faculty of 1000.

Articles (25)

Carrier Protein Mediated Cargo Sensing in Quorum Signal Synthases (Abu Sayeed Chowdhury, Shibani Basu, Eric Baggs, et al.) Chemical Communications (2023)

Acyl-homoserine lactone synthases make specific AHL quorum sensing signals to aid virulence in Gram-negative bacteria. Here, we use solution NMR spectroscopy to demonstrate that the carrier protein–enzyme interface accurately reveals substrate recognition mechanisms in two ...

Expression and Purification of a Cleavable Recombinant Fortilin from Escherichia coli for Structure Activity Studies (Maranda S. Cantrell, Jackson D. Wall, Xinzhu Pu, et al.) Protein Expression and Purification (2022)

Complications related to atherosclerosis account for approximately 1 in 4 deaths in the United States and treatment has focused on lowering serum LDL-cholesterol levels with statins. However, approximately 50% of those diagnosed with atherosclerosis have ...

Bioactive Recombinant Human Oncostatin M for NMR-Based Screening in Drug Discovery (Olga A. Mass, Joseph Tuccinardi, Luke Woodbury, et al.) Scientific Reports (2021)

Oncostatin M (OSM) is a pleiotropic, interleukin-6 family inflammatory cytokine that plays an important role in inflammatory diseases, including inflammatory bowel disease, rheumatoid arthritis, and cancer progression and metastasis. Recently, elevated OSM levels have been ...

Repurposing Drugs to Treat Heart and Brain Illness (Maranda S. Cantrell, Alejandro Soto-Avellaneda, Jackson D. Wall, et al.) Pharmaceuticals (2021)

Drug development is a complicated, slow and expensive process with high failure rates. One strategy to mitigate these factors is to recycle existing drugs with viable safety profiles and have gained Food and Drug Administration ...

Center of Biomedical Research Excellence in Matrix Biology: Building Research Infrastructure, Supporting Young Researchers, and Fostering Collaboration (Julia Thom Oxford, Ken A. Cornell, Jared J. Romero, et al.) International Journal of Molecular Sciences (2020)

The Center of Biomedical Research Excellence in Matrix Biology strives to improve our understanding of extracellular matrix at molecular, cellular, tissue, and organismal levels to generate new knowledge about pathophysiology, normal development, and regenerative medicine. ...

Ribbon α-Conotoxin KTM Exhibits Potent Inhibition of Nicotinic Acetylcholine Receptors (Leanna A. Marquart, Matthew W. Turner, Lisa R. Warner, et al.) Marine Drugs (2019)

KTM is a 16 amino acid peptide with the sequence WCCSYPGCYWSSSKWC. Here, we present the nuclear magnetic resonance (NMR) structure and bioactivity of this rationally designed α-conotoxin (α-CTx) that demonstrates potent inhibition of rat α3β2-nicotinic ...

Flexibility in the Periplasmic Domain of BamA Is Important for Function (Lisa A. Warner, Petia Z. Gatzeva-Topalova, Pamela A. Doerner, et al.) Structure (2017)

The β-barrel assembly machine (BAM) mediates the biogenesis of outer membrane proteins (OMPs) in Gram-negative bacteria. BamA, the central BAM subunit composed of a transmembrane β-barrel domain linked to five polypeptide transport-associated (POTRA) periplasmic domains, ...

The Effects of High Concentrations of Ionic Liquid on GB1 Protein Structure and Dynamics Probed by High-Resolution Magic-Angle-Spinning NMR Spectroscopy (Lisa Warner, Erica Gjersing, Shelby E. Follett, et al.) Biochemistry and Biophysics Reports (2016)

Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid ...

Structural Basis for the Recognition of Spliceosomal SmN/B/B' Proteins by the RBM5 OCRE Domain in Splicing Regulation (André Mourão, Sophie Bonnal, Komal Soni, et al.) eLIFE (2016)

The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing ...

Laboratory Instrumentation: An Exploration of the Impact of Instrumentation on Student Learning (Don L. Warner, Eric C. Brown and Susan E. Shadle) Journal of Chemical Education (2016)

Academic programs generally work to make their laboratory curriculum both as instrumentation rich and up to date as possible. However, little is known about the relationship between the use of instrumentation in the curriculum and ...

Modification of Cellular DNA by Synthetic Aziridinomitosenes (Chris M. Mallory, Ryan P. Carfi, SangPhil Moon, et al.) Bioorganic & Medicinal Chemistry (2015)

Two synthetic aziridinomitosenes (AZMs), Me-AZM and H-AZM, structurally related to mitomycin C (MC) were evaluated for their anticancer activity against six cancer cell lines (HeLa, Jurkat, T47D, HepG2, HL-60, and HuT-78) and tested for their ...

Structure-Based Assignment of Ile, Leu, and Val Methyl Groups in the Active and Inactive Forms of the Mitogen-Activated Protein Kinase Extracellular Signal-Regulated Kinase 2 (Yao Xiao, Lisa Warner, Michael P. Latham, et al.) Biochemistry (2015)

Resonance assignments are the first step in most NMR studies of protein structure, function, and dynamics. Standard protein assignment methods employ through-bond backbone experiments on uniformly <sup>13</sup>C/<sup>15</sup>N-labeled proteins. For larger proteins, this through-bond assignment procedure ...

Transient Electrostatic Interactions Dominate the Conformational Equilibrium Sampled by Multidomain Splicing Factor U2AF65: A Combined NMR and SAXS Study (Lisa R. Warner) Journal of the American Chemical Society (2014)

Multidomain proteins containing intrinsically disordered linkers exhibit large-scale dynamic modes that play key roles in a multitude of molecular recognition and signaling processes. Here, we determine the conformational space sampled by the multidomain splicing factor ...

Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy (Franz Schilling, Lisa R. Warner, Naum I. Gershenzon, et al.) Angewandte Chemie (2014)

Ultra-high-field NMR spectroscopy requires an increased bandwidth for heteronuclear decoupling, especially in biomolecular NMR applications. Composite pulse decoupling cannot provide sufficient bandwidth at practical power levels, and adiabatic pulse decoupling with sufficient bandwidth is compromised ...

Modeling SN2 and E2 Reaction Pathways and Other Computational Exercises in the Undergraduate Organic Chemistry Laboratory (Clifford M. Csizmar, Jeremy P. Daniels, Lauren E. Davis, et al.) Journal of Chemical Education (2013)

Computational chemistry techniques have become increasingly important tools for chemists seeking to address scientific questions. As such, it is important that undergraduate chemistry students develop competence in this emerging field of chemistry. One strategy to ...

Predicted Structure and Binding Motifs of Collagen a1(Xi) (Julia Oxford, Owen McDougal, Lisa Warner, et al.) Journal on Bioinformatics and Biotechnology (2011)

The amino propeptide of collagen a1(XI) (NPP) has been shown to bind glycosaminoglycans and to form a dimer. While these are independent biochemical events, it is likely that dimerization facilitates the interaction with glycosaminoglycans or ...

Structure of the BamC Two-Domain Protein Obtained by Rosetta with a Limited NMR Data Set (Lisa R. Warner, Krisztina Varga, Oliver F. Lange, et al.) Journal of Molecular Biology (2011)

The CS-RDC-NOE Rosetta program was used to generate the solution structure of a 27-kDa fragment of the Escherichia coli BamC protein from a limited set of NMR data. The BamC protein is a component of ...

Partners in Crime: The TGFβ and MAPК Pathways in Cancer Progression (Douglas A. Chapnick, Lisa Warner, Jennifer Bernet, et al.) Cell & Bioscience (2011)

The TGFβ and Ras-MAPК pathways play critical roles in cell development and cell cycle regulation, as well as in tumor formation and metastasis. In the absence of cellular transformation, these pathways operate in opposition to ...

Structure and Flexibility of the Complete Periplasmic Domain of BamA: The Protein Insertion Machine of the Outer Membrane (Petia Zvezdanova Gatzeva-Topalova, Lisa Rosa Warner, Arthur Pardi, et al.) Structure (2010)

Folding and insertion of β-barrel outer membrane proteins (OMPs) is essential for Gram-negative bacteria. This process is mediated by the multiprotein complex BAM, composed of the essential β-barrel OMP BamA and four lipoproteins (BamBCDE). The ...

Characterization of Collagenous Matrix Assembly in a Chondrocyte Model System (Sorcha Yingst, Kaci Bloxham, Lisa R. Warner, et al.) Journal of Biomedical Materials Research Part A (2009)

Collagen is a major component of the newly synthesized pericellular microenvironment of chondrocytes. Collagen types II, IX, and XI are synthesized and assembled into higher ordered complexes by a mechanism in which type XI collagen ...

Calpain-Cleavage of α-Synuclein: Connecting Proteolytic Processing to Disease-Linked Aggregation (Brian M. Dufty, Lisa R. Warner, Kristen M. Leenhouts, et al.) The American Journal of Pathology (2007)

Parkinson's disease (PD) and dementia with Lewy bodies (DLB) are both characterized pathologically by the presence of neuronal inclusions termed Lewy bodies (LBs). A common feature found in LBs are aggregates of α-synuclein (α-Syn), and ...

Expression, Purification, and Refolding of Recombinant Collagen α1(Xi) Amino Terminal Domain Splice Variants (Lisa R. Warner, Christina M. Blasick, Raquel J. Brown, et al.) Protein Expression and Purification (2007)

The amino terminal domain of collagen type XI α1 chain is a noncollagenous structure that is essential for the regulation of fibrillogenesis in developing cartilage. The amino terminal domain is alternatively spliced at the mRNA ...

Isoform-Specific Heparan Sulfate Binding within the Amino-Terminal Noncollagenous Domain of Collagen α1(XI) (Lisa R. Warner, Raquel J. Brown, Sorcha M.C. Yingst, et al.) The Journal of Biological Chemistry (2006)

Collagen type XI is a constituent of the pericellular matrix of chondrocytes and plays a role in the regulation of fibrillogenesis. The amino-terminal domain of collagen type XI α1 chain is a noncollagenous structure that ...

Comparison of the Effects of Arsenic (V), Cadmium (II), and Mercury (II) Single Metal and Mixed Metal Exposure in Radish, Raphanus sativus, Fescue Grass, Festuca ovina, and Duckweed, Lemna minor (H. A. Charlier, C. Albertson, C. Thornock, et al.) Bulletin of Environmental Contamination and Toxicology (2005)

A number of abandoned mine sites in the Boise National Forest have been shown to be contaminated with mixtures of heavy metals including Hg, Cd, As, and Pb (Gillerman et al. 1999; Ellis and Eslick ...

Structural Model of the Amino Propeptide of Collagen XI α1 Chain with Similarity to the LNS Domains (Arzhang Fallahi, Becky Kroll, Lisa R. Warner, et al.) Protein Science (2005)

Fibrillar collagens are the principal structural molecules of connective tissues. The assembly of collagen fibrils is regulated by quantitatively minor fibrillar collagens, types V and XI. A unique amino-terminal propeptide domain of these collagens has ...