My laboratory studies two aspects of lymphocyte development and diversification. One area of active investigation in my laboratory is elucidation of the molecular events required for the induction of apoptosis in the mouse thymus. Negative selection (the removal of autoreactive T cells) occurs by an active cell death process known as apoptosis. To isolate genes that are required for this process, we constructed a cDNA library from dying thymocytes and isolated several genes that are induced or repressed during apoptosis. We have shown that several of these genes, such as p53 and Nur77, are required for cell death. Using techniques such as yeast two-hybrid analysis, we are now asking how these genes mediate cell death in T cells. Our two hybrid screen with Nur77 revealed that Notch-1 interacts with Nur77 and blocks Nur77 dependent apoptosis. Currently we are investigating the mechanism by which Notch-1 blocks Nur77 induced death. The identification of Notch as an interaction partner of Nur77 lead us to further explore the role of Notch in T cell function. Recent data from the lab also has shown that signaling through the T cell receptor in peripheral T lymphocytes induces Notch-1 expression and this regulation of Notch expression in T cells plays an important and critical role in the function of various T cell subsets. We have found that Notch signaling is required for the development of Th1 cells. Th1 cells are known to play a role in several autoimmune diseases and in vivo blockade of Notch signaling blocks the induction of EAE, a murine disease with many of the characteristics of multiple sclerosis. Activation of Notch requires the enzyme g-secretase and current work is focused on the use of a g-secretase inhibitor to study how blockade of Notch signaling affects normal immune function.
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Notch signals in the endothelium and cancer “stem-like” cells: opportunities for cancer therapy (with Jian-Wei Gu, Paola Rizzo, Antonio Pannuti, Todd Golde, and Lucio Miele), Vascular Cell (2012)
Anti-angiogenesis agents and the identification of cancer stem-like cells (CSC) are opening new avenues for...
Notch signaling during peripherl T-cell activation and differentiation, Nature Reviews Immunology (2007)
For many years, researchers have focused on the contribution of Notch signalling to lymphoid development....
Notch signaling in cancer, Current Molecular Medicine (2006)
The evolutionarily conserved developmental pathway driven by Notch receptors and ligands has acquired multiple post-natal...
Defects of immune regulation in the presenilin-1 mutant knock-in mouse (with Grant A. Morgan, Qing Guo, Sic L. Chan, Devin S. Gary, and Mark P. Mattson), Neuromolecular Medicine (2006)
Mutations in the presenilin-1 (PS1) gene are causally linked to early-onset Alzheimer's disease (AD). Studies...
ã-secretase inhibitors block in vivo and in vitro Th1 polarization by preventing Notch upregulation of t-bet, Nature Immunology (2005)
Notch receptors are processed by gamma-secretase acting in synergy with T cell receptor signaling to...