Presentation
Synergistic antimicrobial activity of bovine lactoferrin and cannabidiol (CBD) isolate from hemp (Cannabis sativa) against foodborne pathogens
American Dairy Science Association Annual Meeting
(2023)
Abstract
Foodborne illnesses give rise to numerous health problems every year worldwide. The aim of this study was to determine the antimicrobial activity of bovine lactoferrin in combination with cannabidiol (CBD) isolate from hemp (Cannabis sativa) against Escherichia coli and Staphylococcus aureusin trypticase soy broth. A broth microdilution assay using optical density to monitor bacterial growth was used to determine the synergistic antimicrobial activity of bovine lactoferrin and CBD isolate against the two foodborne pathogens. Effective combinations of these antimicrobial substances were further evaluated for their bactericidal activity by monitoring growth over time using the pour plate method. The data of time kill assays in the growth medium were evaluated by ANOVA and the means were separated using the Tukey’s test to determine significant differences (P < 0.05). The results indicated a difference (P < 0.05) in the growth of these bacteria. Bovine lactoferrin at a Concentration of 500 μg/ml was significantly (P <0.05) inhibitory to 3 strains of Staphylococcus aureus. When bovine lactoferrin was combined with 264 μg/ml of CBD isolate, the minimum inhibitory concentration (MIC) for the 3 strains of Staphylococcus aureus decreased by 3-folds. Synergistic bactericidal activity of bovine lactoferrin and CBD isolate caused significant (P < 0.05) inhibition of the two strains of Escherichia coli. These results indicated that the use of bovine lactoferrin in combination with CBD isolate from hemp may preserve food and beverage products against Escherichia coli and Staphylococcus aureus pathogens.
Keywords
- bovine lactoferrin,
- cannabidiol,
- food pathogens,
- antimicrobial
Disciplines
Publication Date
Summer June 25, 2023
Location
Ottawa, Ontario, Canada
Citation Information
Adela Mora-Gutierrez, Rahmat Attaie, Maryuri Nunez de Gonzalez, Sela Woldesenbet, et al.. "Synergistic antimicrobial activity of bovine lactoferrin and cannabidiol (CBD) isolate from hemp (Cannabis sativa) against foodborne pathogens" American Dairy Science Association Annual Meeting (2023) Available at: http://works.bepress.com/maryuri-nunezdegonzalez/37/