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Presentation
Application of 13C Broadband Pulses to Excitation, Refocusing and Decoupling for High Field
35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies, [Frauenchiemsee Island, Germany] (2013)
  • Lisa Warner, Bayerisches NMR-Zentrum
  • Franz Schilling, Bayerisches NMR-Zentrum
  • Thomas Skinner, Wright State University
  • Steffen Glaser, Bayerisches NMR-Zentrum
Abstract
The <sup>13</sup>C chemical shifts of carbons found in proteins and nucleic acids span a frequency bandwidth that ranges from 22.5 kHz at 14.1 T (600 MHz <sup>1</sup>H) to 45 kHz at 28.2 Tesla(1.2 GHz <sup>1</sup>H). At higher magnetic fields, the conventional pulses used for excitation, refocusing and decoupling fail to cover the full bandwidth within the power limits of modern probes, thus decreasing the ability to excite all nuclei and to decouple multiplet splitting of peaks. Adiabatic broadband decoupling pulses provide lowpower broadband decoupling, but are plagued with intense sideband artifacts. As the spectrometer fields increase, the need for a robust, low power, broadband <sup>13</sup>C decoupling pulse becomes greater. Here, we present low power <sup>13</sup>C decoupling pulses designed with optimal control (OC) theory and broadband refocusing pulses that cover greater than 47 kHz. We show the advantages of using low power OC decoupling scheme and broadband refocusing pulses in <sup>1</sup>H, <sup>13</sup>C HMQC and <sup>13</sup>C-edited <sup>1</sup>H, <sup>1</sup>H NOESY spectra. The OC decoupling pulses are compared with GARP and WURST. Further, we propose the use of these broadband pulses in a suite of optimized pulse sequences intended for <sup>1</sup>H, <sup>13</sup>C HMQC and <sup>13</sup>C- edited/filtered <sup>1</sup>H, <sup>1</sup>H NOESY and <sup>13</sup>C, <sup>13</sup>C NOESY spectra collected at high fields. 
Publication Date
September 10, 2013
Citation Information
Lisa Warner, Franz Schilling, Thomas Skinner and Steffen Glaser. "Application of 13C Broadband Pulses to Excitation, Refocusing and Decoupling for High Field" 35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies, [Frauenchiemsee Island, Germany] (2013)
Available at: http://works.bepress.com/lisa-warner/5/