Current studies on the defense against chronic fibrous have focused on the inhibition of the inflammatory pathways. However, it is believed that an alternative treatment method could be found by inhibiting the overexpression of collagen. A new defense pathway could be discovered by analyzing collagen production and its relationship with a proposed chaperone protein, LARP6. The production of the collagen 1α1(I), 1α2(I), 3α1(I), and 5α2(I) proteins is facilitated by RNA strains containing a highly conserved 5' Stem Loop (5'SL) in the untranslated region. It is believed that LARP6 helps assist in folding the RNA into the stem-loop conformation at which point protein synthesis can initiate. To better understand the effect of LARP6 on collagen production, a deeper understanding of the RNA 5'SL’s 3D structure must be determined. However, prior to analysis, a large stock of purified RNA must be transcribed and isolated. This research project tested a series of collagen RNA transcription reactions under various constraints. By optimizing the transcription conditions, large-scale reactions of collagen RNA can be performed with a high yield. After a large volume of RNA is transcribed, the 5' SL analysis can proceed by nuclear magnetic resonance (NMR).