Breast and prostate tumors can be surgically removed in their earlier stages. The next stage of cancer involves metastasis when a tumor migrates to another area of the body. Metastasis can be triggered by an inflammation response, including surgical intervention. An inflammatory cytokine protein (IC) has been identified as a primary signal in metastasis. By targeting the IC with a small molecule inhibitor (SMI), metastasis can be prevented. Our project aims to develop a protocol for the purification of the inflammatory cytokine protein in milligram quantities. Producing milligram amounts of the protein is essential for nuclear magnetic resonance (NMR) based experiments and small molecule screening assays. Ongoing experiments aim to refine the protocol to further increase the yield of pure, bioactive protein.