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Fluprostenol-Induced MAPK Signaling is Independent of Aging in Fischer 344/NNiaHSd x Brown Norway/BiNia Rat Aorta
MIIR Faculty Research
  • Kevin M. Rice, Marshall University
  • Ernest M. Walker, Marshall University
  • Sunil K. Kakarla, Marshall University
  • Satyanarayana Paturi, Marshall University
  • Miaozong Wu, Marshall University
  • Sumit Narula, Marshall University
  • Eric R. Blough, Marshall University
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Publication Date
The factors that regulate vascular mechanotransduction and how this process may be altered with aging are poorly understood and have not been widely studied. Recent data suggest that increased tissue loading can result in the release of prostaglandin F2 alpha (PGF2α) and other reports indicate that aging diminishes the ability of the aged aorta to activate mitogen activated protein kinase (MAPK) signaling in response to increased loading. Using ex vivo incubations, here we investigate whether aging affects the ability of the aorta to induce phosphorylation of extracellular signal-regulated kinase 1/2 (ERK½-MAPK), p38-MAPK, and Jun N-terminal kinase (JNK-MAPK) activation following stimulation with a PGF2α analog, fluprostenol. Compared to aortas from 6-mo animals, the amounts of ERK½- and p38-MAPK remained unchanged with aging, while the level of JNK-MAPK protein increased by 135% and 100% at 30- and 36-mo, respectively. Aging increased the basal phosphorylation of ERK½ (115% and 47%) and JNK (29% and 69%) (p <0.05) in 30- and 36-mo aortas, while p38 phosphorylation levels remained unaltered. Compared to age-matched controls, fluprostenol induced phosphorylation of ERK½ (310%, 286%, and 554%), p38-MAPK (unchanged, 48%, and 148%), and JNK (78%, 88%, and 95%) in 6-, 30- and 36-mo aortas, respectively. These findings suggest that aging does not affect the ability of the rat aorta to activate ERK½-, p38-MAPK, and JNK-MAPK phosphorylation in response to PGF2α stimulation.

This article first appeared in the winter 2010 issue of Annals of Clinical & Laboratory Science, the member magazine of the Association of Clinical Scientists, Inc., and is reprinted with permission.

© 2010 by the Association of Clinical Scientists, Inc.

Citation Information
Rice KM, Walker EM, Kakarla SK, Paturi S, Wu M, Narula S. Blough ER (2010) Fluprostenol-induced MAPK Signaling is independent of aging in the Fischer 344/NNiaHSd X Brown Norway/BiNia Rat Aorta. Ann Clin Lab Sci 40:26-31.