Kola nuts were regularly chewed by West Africans and Beninese in particularly. The aim of this

study was to investigate nutritional and anti-nutrient content of three Benin’s kola nuts (Cola nitida,

Cola acuminata and Garcinia kola). Proximate composition of the three species of kola nuts

was assessed using standard analytical AOAC methods. Phenolics and flavonoids contents were

determined by Folin-Ciocalteu and aluminum trichloride methods, respectively. Mineral composition

was determined by Atomic Absorption Spectrometry method. Free and total amino acids were

separated and quantified by HPLC. Protein content of the three kola nuts ranges from 4.95% (G.

kola) to 10.64% (C. acuminata) whereas fat content ranges from 0.2 ± 0.00 (C. nitida) to 2.5 ± 0.42

(G. kola). Total phenolics abounded (2444.96 ± 81.56 μg Eq AG/100g) in C. acuminata, while flavonoids

predominated (561.69 ± 22.10 μgEqQ/100g) in G. kola. The three species are a good source of magnesium and a copper provider was lowest in C. nitida (0.59 ± 0.08 mg/g) and in C. acuminata (0.65 ± 0.02 mg/g). The dominant total essential amino acids were threonine (C. acuminata) and methionine (C. acuminata and G. kola), while the predominant non-essential total amino acids according to species were arginine (C. nitida and G. kola), proline (C. acuminata) and cysteine (G. kola). For the anti-nutrients factors, saponins were in great proportion (8.33% ± 0.25%), while the oxalates were in small proportion (0.44% ± 0.04%). The three species have an interesting nutritional composition, but these seeds have the relatively lowest amino acids content.