Skip to main content
Article
T-705 (Favipiravir) Inhibition of Arenavirus Replication in Cell Culture
Antimicrobial Agents and Chemotherapy
  • Michelle Mendenhall
  • Andrew Russell
  • Terry Juelich
  • Emily L. Messina
  • Donald F. Smee
  • Alexander N. Freiberg
  • Michael R. Holbrook
  • Yousuke Furuta
  • Juan-Carlos de la Torre
  • Jack H. Nunberg, University of Montana - Missoula
  • Brian B. Gowen
Document Type
Article
Publication Date
2-1-2011
Disciplines
Abstract

A number of New World arenaviruses (Junin [JUNV], Machupo [MACV], and Guanarito [GTOV] viruses) can cause human disease ranging from mild febrile illness to a severe and often fatal hemorrhagic fever syndrome. These highly pathogenic viruses and the Old World Lassa fever virus pose a significant threat to public health and national security. The only licensed antiviral agent with activity against these viruses, ribavirin, has had mixed success in treating severe arenaviral disease and is associated with significant toxicities. A novel pyrazine derivative currently in clinical trials for the treatment of influenza virus infections, T-705 (favipiravir), has demonstrated broad-spectrum activity against a number of RNA viruses, including arenaviruses. T-705 has also been shown to be effective against Pichinde arenavirus infection in a hamster model. Here, we demonstrate the robust antiviral activity of T-705 against authentic highly pathogenic arenaviruses in cell culture. We show that T-705 disrupts an early or intermediate stage in viral replication, distinct from absorption or release, and that its antiviral activity in cell culture is reversed by the addition of purine bases and nucleosides, but not with pyrimidines. Specific inhibition of viral replication/transcription by T-705 was demonstrated using a lymphocytic choriomeningitis arenavirus replicon system. Our findings indicate that T-705 acts to inhibit arenavirus replication/transcription and may directly target the viral RNA-dependent RNA polymerase.

DOI
10.1128/AAC.01219-10
Citation Information
Michelle Mendenhall, Andrew Russell, Terry Juelich, Emily L. Messina, et al.. "T-705 (Favipiravir) Inhibition of Arenavirus Replication in Cell Culture" Antimicrobial Agents and Chemotherapy Vol. 55 Iss. 2 (2011) p. 782 - 787
Available at: http://works.bepress.com/brian_gowen/37/