Article
Biosynthesis of silver nanoparticles using extract of Rumex nepalensis for bactericidal effect against food-borne pathogens and antioxidant activity
Frontiers in Molecular Biosciences
(2022)
Abstract
The evolution and incidence of multidrug-resistant food-borne pathogens still
become a critical public health global issue. To avert this challenge there is great
interest in medical applications of silver nanoparticles. Thus, this study aimed to
synthesize silver nanoparticles (Rn-AgNPs) using aqueous leaf extract of Nepal
Dock (Rumex nepalensis Spreng) and evaluate their antibacterial potential
against food-borne pathogens and antioxidant activity. The Rn-AgNPs were
characterized by UV-Vis spectrophotometry, Dynamic Light Scattering (DLS),
Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red
Spectroscopy (FTIR). The antibacterial activities of the Rn-AgNPs were
evaluated using agar well diffusion (zone of inhibition, ZOI) and
microdilution (minimum inhibitory concentration, MIC and minimum
bactericidal concentration, MBC) methods. The antioxidant property of the
Rn-AgNPs was investigated using radical scavenging (DPPH and hydroxyl)
assays. The UV-Vis spectra of Rn-AgNPs elucidated the absorption maxima
at 425 nm and FTIR detected numerous functional groups of biological
compounds that are responsible for capping and stabilizing Rn-AgNPs. DLS
analysis displayed monodispersed Rn-AgNPs of 86.7 nm size and highly
negative zeta potential (-32.5 mV). Overall results showed that Escherichia
coli was the most sensitive organism, whereas Staphylococcus aureus was
the least sensitive against Rn-AgNPs. In the antioxidant tests, the AgNPs radical
scavenging activity reached 95.44% at 100 μg/ml. This study indicates that Rn-
AgNPs exhibit a strong antimicrobial on L. monocytogenes, S. aureus, S.
typhimurium, and E. coli and antioxidant and thus might be developed as a
new type of antimicrobial agent for the treatment of multidrug-resistant
foodborne pathogens and extensible applications in nanomaterial food- and
nanocomposite-based antimicrobial packaging and/or as an antioxidant.
Keywords
- Rumex nepalensis,
- Rn-AgNPs,
- antimicrobial,
- antioxidant,
- foodborne,
- drug-resistant
Disciplines
Publication Date
Fall September 20, 2022
DOI
10.3389/fmolb.2022.991669
Citation Information
Addisie Geremew, Laura Carson and Selamawit Woldesenbet. "Biosynthesis of silver nanoparticles using extract of Rumex nepalensis for bactericidal effect against food-borne pathogens and antioxidant activity" Frontiers in Molecular Biosciences Vol. 9 (2022) p. 1 - 16 Available at: http://works.bepress.com/addisie-geremew/8/
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