Presentation
Green Syntheses and Characterizations of Silver Nanoparticles for Food Safety Applications
Cell Bio, American Society for Cell Biology (ASCB)
(2022)
Abstract
There is a growing demand and application of nanoparticles in various industries such as electronics,
cosmetics, food safety, optics, and medicine. Green synthesis of silver nanoparticles (AgNPs) using
medicinal plants has been investigated due to the presence of phytochemicals that play a role in the
reduction process. Thus, this study aimed to synthesize and characterize AgNPs using aqueous leaf
extracts of Salvia blepharophylla and Salvia greggii, and to assess their antimicrobial activity on the
multidrug-resistant species of bacteria, Escherichia coli, Salmonella typhimurium, Listeria, and
Staphylococcus aureus. The formation of AgNPs was confirmed using UV-vis spectroscopy as the
reaction solution changed from a light brown to a darker brown. The particle stability and sizes were
determined using the Anton Paar Litesizer. Agar well diffusion method was used to measure zones of
inhibition (ZOI) to determine the antimicrobial properties of the AgNPs. Preliminary data indicated that
AgNPs sizes were between 80-92 nm, and 80-84 nm, using extract of S. blepharophylla and S. greggii,
respectively. Additionally, zeta potentials of AgNPs synthesized using S. blepharophylla ranged from -23
to -26 mV, and -20 to -25 mV using S. greggii. Overall results showed that E. coli, S. typhimurium,
Listeria, and S. aureus were inhibited by AgNPs synthesized using the extract of S. blepharophylla with
ZOIs were 0.9-1.2 mm, 0.9-1.1 mm, 0.3-1.3 mm, and 0.95-1.25 mm, respectively. Additionally, E. coli, S.
typhimurium, Listeria, and S. aureus were inhibited by AgNPs synthesized using the extract of S. greggii
ZOIs were 1-1.15 mm, 0.9-1.3 mm, 1-1.4 mm, and 1-1.3 mm, correspondingly. S. aureus seemed to be
the most sensitive bacteria overall, against both plant-based AgNPs.
Disciplines
Publication Date
Winter December 3, 2022
Location
Washington, DC
Citation Information
Addisie Geremew. "Green Syntheses and Characterizations of Silver Nanoparticles for Food Safety Applications" Cell Bio, American Society for Cell Biology (ASCB) (2022) Available at: http://works.bepress.com/addisie-geremew/12/