Insufficient levels of physical activity have contributed to the epidemic of childhood obesity in the United States. After-school based physical activity programs may positively impact this problem. The purpose of this 12-week study was to describe the implementation and evaluation of a National Institute on Out of School Time standards based after-school physical activity program on health-related fitness variables in elementary school children (n=14) ranging in age from 5-11 years old. Children participated twice per week for 60 minutes each session. Physical activities included Geomotion mats, sport stacking cups, and outdoor activities. Data analysis was done using the statistical software Statistical Package for the Social Sciences (SPSS, version 17.0). Significant reductions in body fat percentage were found along with significant increases in height and body weight. Although the results of this program were overall positive, future research is still needed to reexamine the effect a NIOST program can have on the physical fitness levels of students. More research is needed to determine the feasibility of replicating this program.