Article
The Preformulation Stability Studies of a Proline Prodrug of Methotrexate
Medicinal Chemistry
(2012)
Abstract
In order to reduce toxicity of methotrexate and improve bioavailability, permeability, and explore other delivery routes, a proline prodrug of methotrexate was synthesized and preformulation stability studies were conducted under accelerated conditions to assess compound liability and possible conversion to the parent drug. Forced degradation studies showed that the prodrug will degrade in the presence of water, acid, and heat (70 °C), generating the parent compound methotrexate. It was also found that this conversion is temperature dependent. In addition, the prodrug is extremely light and oxidative labile. Therefore, future formulation studies should be light protected and stabilized by a suitable antioxidant. It was also found that the prodrug is stable in the HPLC diluent, consisting of water and acetonitrile; stored bench-top and protected from light for up to two weeks.
Keywords
- Preformulation,
- Stability,
- Forced degradation,
- Hydrolysis,
- Methotrexate,
- Proline,
- Prodrug,
- MTX,
- acetonitrile,
- Amethopterin
Disciplines
Publication Date
2012
DOI
https://doi.org/10.2174/157340612801216166
Citation Information
Ann Stephenson, Zhiqian (James) Wu and Xudong Yuan. "The Preformulation Stability Studies of a Proline Prodrug of Methotrexate" Medicinal Chemistry Vol. 8 Iss. 4 (2012) Available at: http://works.bepress.com/zhiqianjames_wu/17/