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Article
The HP1 homolog rhino anchors a nuclear complex that suppresses piRNA precursor splicing
Program in Bioinformatics and Integrative Biology Publications and Presentations
  • Zhao Zhang, University of Massachusetts Medical School
  • Jie Wang, University of Massachusetts Medical School
  • Nadine McGinnis-Schultz, University of Massachusetts Medical School
  • Fan Zhang, University of Massachusetts Medical School
  • Swapnil S. Parhad, University of Massachusetts Medical School
  • Shikui Tu, University of Massachusetts Medical School
  • Thom Vreven, University of Massachusetts Medical School
  • Phillip D. Zamore, University of Massachusetts Medical School
  • Zhiping Weng, University of Massachusetts Medical School
  • William E. Theurkauf, University of Massachusetts Medical School
UMMS Affiliation
Program in Molecular Medicine; Program in Bioinformatics and Integrative Biology; Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Date
6-5-2014
Document Type
Article
Medical Subject Headings
Animals; Chromosomal Proteins, Non-Histone; DEAD-box RNA Helicases; Drosophila Proteins; Drosophila melanogaster; Female; Ovary; *RNA Splicing; RNA, Small Interfering; RNA-Binding Proteins; SOXD Transcription Factors
Abstract
piRNAs guide an adaptive genome defense system that silences transposons during germline development. The Drosophila HP1 homolog Rhino is required for germline piRNA production. We show that Rhino binds specifically to the heterochromatic clusters that produce piRNA precursors, and that binding directly correlates with piRNA production. Rhino colocalizes to germline nuclear foci with Rai1/DXO-related protein Cuff and the DEAD box protein UAP56, which are also required for germline piRNA production. RNA sequencing indicates that most cluster transcripts are not spliced and that rhino, cuff, and uap56 mutations increase expression of spliced cluster transcripts over 100-fold. LacI::Rhino fusion protein binding suppresses splicing of a reporter transgene and is sufficient to trigger piRNA production from a trans combination of sense and antisense reporters. We therefore propose that Rhino anchors a nuclear complex that suppresses cluster transcript splicing and speculate that stalled splicing differentiates piRNA precursors from mRNAs.
Rights and Permissions
Citation: Cell. 2014 Jun 5;157(6):1353-63. doi: 10.1016/j.cell.2014.04.030. Link to article on publisher's site
Comments

Co-authors Zhao Zhang and Fan Zhang are both doctoral students in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources
Link to Article in PubMed
PubMed ID
24906152
Citation Information
Zhao Zhang, Jie Wang, Nadine McGinnis-Schultz, Fan Zhang, et al.. "The HP1 homolog rhino anchors a nuclear complex that suppresses piRNA precursor splicing" Vol. 157 Iss. 6 (2014) ISSN: 0092-8674 (Linking)
Available at: http://works.bepress.com/zhao-zhang/8/