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Article
UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery
Program in Bioinformatics and Integrative Biology Publications and Presentations
  • Fan Zhang, University of Massachusetts Medical School
  • Jie Wang, University of Massachusetts Medical School
  • Jia Xu, University of Massachusetts Medical School
  • Zhao Zhang, University of Massachusetts Medical School
  • Birgit S. Koppetsch, University of Massachusetts Medical School
  • Nadine Schultz, University of Massachusetts Medical School
  • Thom Vreven, University of Massachusetts Medical School
  • Carine Meignin, Universite de Strasbourg
  • Ilan Davis, The University of Oxford
  • Phillip D. Zamore, University of Massachusetts Medical School
  • Zhiping Weng, University of Massachusetts Medical School
  • William E. Theurkauf, University of Massachusetts Medical School
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology; Program in Molecular Medicine; Program in Cell and Developmental Dynamics
Date
11-9-2012
Document Type
Article
Medical Subject Headings
Animals; DEAD-box RNA Helicases; DNA Damage; DNA Transposable Elements; Drosophila Proteins; Drosophila melanogaster; Female; Germ Cells; Male; Nuclear Envelope; RNA, Small Interfering
Abstract
piRNAs silence transposons during germline development. In Drosophila, transcripts from heterochromatic clusters are processed into primary piRNAs in the perinuclear nuage. The nuclear DEAD box protein UAP56 has been previously implicated in mRNA splicing and export, whereas the DEAD box protein Vasa has an established role in piRNA production and localizes to nuage with the piRNA binding PIWI proteins Ago3 and Aub. We show that UAP56 colocalizes with the cluster-associated HP1 variant Rhino, that nuage granules containing Vasa localize directly across the nuclear envelope from cluster foci containing UAP56 and Rhino, and that cluster transcripts immunoprecipitate with both Vasa and UAP56. Significantly, a charge-substitution mutation that alters a conserved surface residue in UAP56 disrupts colocalization with Rhino, germline piRNA production, transposon silencing, and perinuclear localization of Vasa. We therefore propose that UAP56 and Vasa function in a piRNA-processing compartment that spans the nuclear envelope.
Rights and Permissions
Citation: Cell. 2012 Nov 9;151(4):871-84. doi: 10.1016/j.cell.2012.09.040. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
23141543
Citation Information
Fan Zhang, Jie Wang, Jia Xu, Zhao Zhang, et al.. "UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery" Vol. 151 Iss. 4 (2012) ISSN: 0092-8674 (Linking)
Available at: http://works.bepress.com/zhao-zhang/15/