|Present||Assistant Professor, Iowa State University ‐ Department of Chemical and Biological Engineering|
Honors and Awards
- National Academies Keck Futures Initiative Award
- CH E 415/515 - Biochemical Engineering
4140 Biorenewables Research Laboratory
Ames, IA 50011
Phone: 515 294-1132
Peer-Reviewed Articles (8)
Electrochemical Conversion of Biologically Produced Muconic Acid: Key Considerations for Scale-Up and Corresponding Technoeconomic Analysis ACS Sustainable Chemistry & Engineering (2016)
Muconic acid, an unsaturated diacid that can be produced from cellulosic sugars and lignin monomers by fermentation, emerges as a promising intermediate for the sustainable manufacture of commodity polyamides and polyesters including Nylon-6,6 and polyethylene ...
Exploiting Issatchenkia orientalis SD108 for succinic acid production Microbial Cell Factories (2014)
Background: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development ...
Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster Nature Communications (2013)
Polycyclic tetramate macrolactams (PTMs) are a widely distributed class of natural products with important biological activities. However, many of these PTMs have not been characterized. Here we apply a plug-and-play synthetic biology strategy to activate ...
Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold ACS Synthetic Biology (2013)
Natural products (secondary metabolites) are a rich source of compounds with important biological activities. Eliciting pathway expression is always challenging but extremely important in natural product discovery because an individual pathway is tightly controlled through ...
Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler Molecular BioSystems (2011)
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements ...
Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis Molecular BioSystems (2010)
Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster ...
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways Nucleic Acids Research (2009)
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in ...
Rational Pathway Engineering of Type I Fatty Acid Synthase Allows the Biosynthesis of Triacetic Acid Lactone from D-Glucose in Vivo Journal of the American Chemical Society (2004)
Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid ...