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About Zengyi Shao

The advent of synthetic biology has revolutionized our ability to discover and construct new biosynthetic pathways and engineer platform organisms, or so-called microbial factories, to produce a wide variety of value-added products. Our laboratory focuses on engineering individual microorganisms as well as microbial consortia to address critical issues in energy sustainability and chemical production.
Engineering Non-Conventional Yeast: We are interested in exploring the potential of non-conventional yeast strains based on their special features that S. cerevisiae does not possess. For example, we study the transcriptomics and metabolomics of Pichia stipitis to understand its cellular metabolism regarding its high capacity of pentose utilization; we use the superior acid tolerance of Issatchenkia orientalis to create a superbug for producing short chain and medium chain fatty acids, the important precursors in industry for synthesizing polymers, surfactants, lubricants and biofuels.
Exploring Microbial Consortia: Microbial consortia, composed of multiple interacting microbial populations, can carry out complicated tasks that are more difficult or even impossible for individual populations to perform. The existence of such cooperation and division of labor are common in nature, where organisms establish mutual relationship. We can easily find such examples e.g. between bacteria for anaerobic methane oxidation, between plants and bacteria for global nitrogen fixation, and in higher animals where gut microbes facilitate food utilization and metabolite transfer. Here we exploit a genetically engineered microbial consortium of multiple yeast strains to address the issue of mixed sugar utilization in lignocellulosic hydrolysates.
Developing Strategies for High-Throughput Strain Optimization: We are interested in designing various protein, pathway and genome engineering strategies to systematically optimize strain performance. One example project is to develop a high-throughput sensing platform to report fatty acid product profiles. Fatty acid synthesis naturally occurs via six recurring reactions with two more carbons added in each cycle. Nowadays, production of fatty acids can easily be achieved through expressing the corresponding biosynthetic pathway in genetically trackable organisms. The challenge is how to produce a relatively pure fatty acid with a defined chain length. The product of natural fatty acid synthesis is usually a mixture of compounds with different chain lengths because the “gatekeeper” thioesterase (TE) hydrolyzes thioester bond promiscuously. In order to identify highly specific TEs to produce “pure” fatty acids, we engineer a series of transcriptional regulators responsive to fatty acids with defined chain lengths, as a high-throughput sensing platform, to report product profiles for the large library of TE variants.

Positions

Present Assistant Professor, Iowa State University Department of Chemical and Biological Engineering
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Research Interests


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Honors and Awards

  • National Academies Keck Futures Initiative Award

Courses

  • CH E 415/515 - Biochemical Engineering

Contact Information

4140 Biorenewables Research Laboratory
Ames, IA 50011
Phone: 515 294-1132

Email:


Peer-Reviewed Articles (8)