Skip to main content
Article
Dopamine D1 receptor-mediated NMDA receptor insertion depends on Fyn but not Src kinase pathway in prefrontal cortical neurons
Molecular Brain
  • Jian-Li Hu
  • Gang Liu
  • Yan-Chun Li
  • Wen-Jun Gao
  • Yueqiao Huang, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2010
Abstract
Background. Interactions between dopamine and glutamate in the prefrontal cortex are essential for cognitive functions such as working memory. Modulation of N-methyl-D-aspartic acid (NMDA) receptor functions by dopamine D1 receptor is believed to play a critical role in these functions. The aim of the work reported here is to explore the signaling pathway underlying D1 receptor-mediated trafficking of NMDA receptors in cultured rat prefrontal cortical neurons. Results. Activation of D1 receptor by selective agonist SKF-81297 significantly increased the expression of NR2B subunits. This effect was completely blocked by small interfering RNA knockdown of Fyn, but not Src. Under control conditions, neither Fyn nor Src knockdown exhibited significant effect on basal NR2B expression. D1 stimulation significantly enhanced NR2B insertion into plasma membrane in cultured PFC neurons, a process obstructed by Fyn, but not Src, knockdown. Conclusions. Dopamine D1 receptor-mediated increase of NMDA receptors is thus Fyn kinase dependent. Targeting this signaling pathway may be useful in treating drug addiction and schizophrenia.
Comments

This article was published in Molecular Brain, Volume 3, Issue 1.

The published version is available at http://dx.doi.org/10.1186/1756-6606-3-20.

Copyright © 2010 Hu and distributed under CC-BY.

Citation Information
Jian-Li Hu, Gang Liu, Yan-Chun Li, Wen-Jun Gao, et al.. "Dopamine D1 receptor-mediated NMDA receptor insertion depends on Fyn but not Src kinase pathway in prefrontal cortical neurons" Molecular Brain Vol. 3 Iss. 1 (2010)
Available at: http://works.bepress.com/yue-qiaogeorge_huang/3/