Article
Coupling Ion Mobility Separations, Collisional Activation Techniques, and Multiple Stages of MS for Analysis of Complex Peptide Mixtures
Analytical Chemistry
(2002)
Abstract
An ion trap/ion mobility/quadrupole/collision cell/timeof-
flight mass spectrometer that incorporates a differentially
pumped orifice-skimmer cone region at the back of
the drift tube has been developed for the analysis of
peptide mixtures. The combined approach allows a variety
of strategies to be employed for collisionally activating
ions, and fragments can be monitored by subsequent
stages of mass spectrometry in a parallel fashion, as
described previously (Anal. Chem. 2000, 72, 2737).
Here, we describe the overall experimental approach in
detail. Applications involving different aspects of the initial
mobility separation and various collisional activation and
parallel sequencing strategies are illustrated by examining
several simple peptide mixtures and a mixture of tryptic
peptides from â-casein. Detection limits associated with
various experimental configurations and the utility for
analysis of complex systems are discussed.
Disciplines
Publication Date
March, 2002
DOI
10.1021/ac010837s
Publisher Statement
Reprinted (adapted) with permission from Analytical Chemistry 74 (2002): 992, doi: 10.1021/ac010837s. Copyright 2002 American Chemical Society.
Citation Information
Cherokee S. Hoaglund-Hyzer, Young Jin Lee, Anne E. Counterman and David E. Clemmer. "Coupling Ion Mobility Separations, Collisional Activation Techniques, and Multiple Stages of MS for Analysis of Complex Peptide Mixtures" Analytical Chemistry Vol. 74 Iss. 5 (2002) p. 992 - 1006 Available at: http://works.bepress.com/youngjin-lee/8/