Article
Development of High-Sensitivity Ion Trap Ion Mobility Spectrometry Time-of-Flight Techniques: A High-Throughput Nano-LC-IMS-TOF Separation of Peptides Arising from a Drosophila Protein Extract
Analytical Chemistry
(2003)
Abstract
A linear octopole trap interface for an ion mobility timeof-
flight mass spectrometer has been developed for focusing
and accumulating continuous beams of ions produced
by electrospray ionization. The interface improves experimental
efficiencies by factors of 50-200 compared with
an analogous configuration that utilizes a three-dimensional
Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee,
Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem.
2002, 74, 992-1006). With these improvements, it is
possible to record nested drift (flight) time distributions
for complex mixtures in fractions of a second. We demonstrate
the approach for several well-defined peptide
mixtures and an assessment of the detection limits is
given. Additionally, we demonstrate the utility of the
approach in the field of proteomics by an on-line, threedimensional
nano-LC-ion mobility-TOF separation of tryptic
peptides from the Drosophila proteome.
Disciplines
Publication Date
October, 2003
DOI
10.1021/ac030107f
Publisher Statement
Reprinted (adapted) with permission from Analytical Chemistry 75 (2003): 5137, doi: 10.1021/ac030107f. Copyright 2003 American Chemical Society.
Citation Information
Sunnie Myung, Young Jin Lee, Myeong Hee Moon, John Taraszka, et al.. "Development of High-Sensitivity Ion Trap Ion Mobility Spectrometry Time-of-Flight Techniques: A High-Throughput Nano-LC-IMS-TOF Separation of Peptides Arising from a Drosophila Protein Extract" Analytical Chemistry Vol. 75 Iss. 19 (2003) p. 5137 - 5145 Available at: http://works.bepress.com/youngjin-lee/6/