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Article
MALDI-MS analysis and imaging of smallmolecule metabolites with 1,5-diaminonaphthalene (DAN)
Journal of Mass Spectrometry
  • Andrew R. Korte, Iowa State University
  • Young-Jin Lee, Iowa State University
Document Type
Article
Publication Date
5-1-2014
DOI
10.1002/jms.3400
Abstract
1,5-diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix-assisted laser desorption ioni- zation-mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metab- olite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9-aminoacridine (9-AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9-AA. Finally, DAN and 9-AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.
Comments

This article is from Journal of Mass Spectrometry 49 (2014): 737, doi: 10.1002/jms.3400 . Posted with permission.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Andrew R. Korte and Young-Jin Lee. "MALDI-MS analysis and imaging of smallmolecule metabolites with 1,5-diaminonaphthalene (DAN)" Journal of Mass Spectrometry Vol. 49 (2014) p. 737 - 741
Available at: http://works.bepress.com/youngjin-lee/26/