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Article
Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ
Current Opinion in Biotechnology
  • Drew Sturtevant, University of North Texas
  • Young-Jin Lee, Iowa State University
  • Kent D. Chapman, University of North Texas
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2-1-2016
DOI
10.1016/j.copbio.2015.10.004
Abstract
Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. It is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation
Comments

NOTICE: This is the author’s version of a work that was accepted for publication in Current Opinion in Biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Current Opinion in Biotechnology, 37, February 2016, doi: 10.1016/j.copbio.2015.10.004.

Copyright Owner
Elsevier Ltd
Language
en
File Format
application/pdf
Citation Information
Drew Sturtevant, Young-Jin Lee and Kent D. Chapman. "Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ" Current Opinion in Biotechnology Vol. 37 (2016) p. 53 - 60
Available at: http://works.bepress.com/youngjin-lee/24/