In this case study, we investigate the responses of Glyphosate-Susceptible and Glyphosate-Resistant Amaranthus palmeri to an organic 10% acetic acid herbicide solution instead of the standard 20% acetic acid solution. We show that although both forms respond differently to glyphosate-based herbicides, both will respond the same way to organic-based herbicides that include acetic acid. After treatment with the 10% acetic acid solution, eighty-five percent (85%) of the Glyphosate-Susceptible plants died within 24 h, while 100% of the Glyphosate-Resistant plants died within 24 h. Using a lesser concentration may be better for the environment since there will be less buildup over time and is more cost effective for the farmer.