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Article
BSA–Boronic Acid Conjugate as Lectin Mimetics
Biochemical and Biophysical Research Communications
  • Satya Nandana Narla, Cleveland State University
  • Poornima Pinnamaneni, Cleveland State University
  • Huan Nie, Harbin Institute of Technology
  • Yu Li, Harbin Institute of Technology
  • Xue-Long Sun, Cleveland State University
Document Type
Article
Publication Date
1-10-2014
Disciplines
Abstract

We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.

Comments

https://doi.org/10.1016/j.bbrc.2013.12.006

The authors acknowledge the Ohio Department of Development (ODOD) for supporting this research through research fund at the Center for Gene Regulation in Health and Disease (GRHD) at Cleveland State University. This work was supported in part by Grants from National Science Foundation MRI Grant (CHE-1126384). This work was partially supported by Grants from The National Natural Science Foundation of China (31328006).

Creative Commons License
Creative Commons Attribution-Noncommercial-No Derivative Works 4.0
DOI
10.1016/j.bbrc.2013.12.006
Version
Postprint
Citation Information
Narla, S. N.; Pinnamaneni, P.; Nie, H.; Li, Y.; Sun, X. BSA–boronic acid conjugate as lectin mimetics. Biochem. Biophys. Res. Commun. 2014, 443, 562-567.