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Specific N-glycans of Hepatocellular Carcinoma Cell Surface and the Abnormal Increase of Core-α-1, 6-fucosylated Triantennary Glycan via N-acetylglucosaminyltransferases-IVa Regulation
Scientific Reports
  • Huan Nie, Harbin Institute of Technology
  • Xia Liu, Harbin Institute of Technology
  • Yubao Zhang, Harbin Medical University
  • Tingting Li, Harbin Institute of Technology
  • Chao Zhan, Harbin Medical University
  • Wenjuan Huo, Harbin Institute of Technology
  • Anshun He, Harbin Institute of Technology
  • Yuanfei Yao, Harbin Institute of Technology
  • Yu Jin, Harbin Medical University
  • Youpeng Qu, Harbin Institute of Technology
  • Xue-Long Sun, Cleveland State University
  • Yu Li, Harbin Institute of Technology
Document Type
Article
Publication Date
1-1-2015
Abstract

Glycosylation alterations of cell surface proteins are often observed during the progression of malignancies. The specific cell surface N-glycans were profiled in hepatocellular carcinoma (HCC) with clinical tissues (88 tumor and adjacent normal tissues) and the corresponding serum samples of HCC patients. The level of core-α-1,6-fucosylated triantennary glycan (NA3Fb) increased both on the cell surface and in the serum samples of HCC patients (p < 0.01). Additionally, the change of NA3Fb was not influenced by Hepatitis B virus (HBV)and cirrhosis. Furthermore, the mRNA and protein expression of N-acetylglucosaminyltransferase IVa (GnT-IVa), which was related to the synthesis of the NA3Fb, was substantially increased in HCC tissues. Knockdown of GnT-IVa leads to a decreased level of NA3Fb and decreased ability of invasion and migration in HCC cells. NA3Fb can be regarded as a specific cell surface N-glycan of HCC. The high expression of GnT-IVa is the cause of the abnormal increase of NA3Fb on the HCC cell surface, which regulates cell migration. This study demonstrated the specific N-glycans of the cell surface and the mechanisms of altered glycoform related with HCC. These findings lead to better understanding of the function of glycan and glycosyltransferase in the tumorigenesis, progression and metastasis of HCC.

Comments

This work was partially supported by grants from The National Natural Science Foundation of China (81171945 and 31328006).

Creative Commons License
Creative Commons Attribution 4.0
DOI
10.1038/srep16007
Version
Publisher's PDF
Citation Information
Nie, H.; Liu, X.; Zhang, Y.; Li, T.; Zhan, C.; Huo, W.; He, A.; Yao, Y.; Jin, Y.; Qu, Y.; Sun, X.; Li, Y. Specific N-glycans of Hepatocellular Carcinoma Cell Surface and the Abnormal Increase of Core-α-1, 6-fucosylated Triantennary Glycan via N-acetylglucosaminyltransferases-IVa Regulation. Scientific Reports 2015, 5, 16007.