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Article
Immune complex-induced, nitric oxide-mediated vascular endothelial cell death by phagocytes is prevented with decoy FcgReceptors
Research Day
  • Ramanjaneya V.R. Mula, Philadelphia College of Osteopathic Medicine
  • Deepah Machiah, Philadelphia College of Osteopathic Medicine
  • Srinivasa R. Dalta, Philadelphia College of Osteopathic Medicine
  • Xinyu Wang, Philadelphia College of Osteopathic Medicine
  • Avadhesh C. Sharma, Philadelphia College of Osteopathic Medicine
  • Periasamy Selvaraj, Philadelphia College of Osteopathic Medicine
  • Rangaiah Shashidharamurthy, Philadelphia College of Osteopathic Medicine
Location
Georgia
Start Date
12-5-2015 1:00 PM
Description

The interaction of Fc gamma receptors (FcγRs) expressed on inflammatory cells and immune-complexes (ICs) results in blood vessel damage during autoimmune vasculitis. Thus we tested if uncoupling these interactions of FcγRs and ICs prevented endothelium damage. Herein, we demonstrate that dimeric FcγR-Igs prevented apoptosis of antibody-coated human umbilical vein endothelial cells (HUVECs) mediated by nitric oxide (NO) released from murine macrophages. FcγR-Igs inhibited the IC-induced upregulation of inducible nitric oxide synthase and NO release by macrophages there by prevented the expression of pro-apoptotic genes in HUVECs. FcgR-Igs did not affect exogenous NO-induced upregulation of pro-apoptotic genes in HUVECs. The co-localization of FcγR-Igs and ICs in the vascular regions of various organs revealed that FcγRs-Igs bind to ICs and prevent vascular endothelial cell death in vivo. In conclusion, these data suggest that IC-induced NO is a major factor promoting blood vessel inflammation and endothelial cell death during IC-mediated vasculitis which can be effectively blocked by dimeric decoy FcγRs.

Citation Information
Ramanjaneya V.R. Mula, Deepah Machiah, Srinivasa R. Dalta, Xinyu Wang, et al.. "Immune complex-induced, nitric oxide-mediated vascular endothelial cell death by phagocytes is prevented with decoy FcgReceptors" (2015)
Available at: http://works.bepress.com/xinyu_wang/12/