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Unmasking imaging forces on soft biological samples in liquids when using dynamic atomic force microscopy: A case study on viral capsids
Birck and NCN Publications
  • Xin Xu, Purdue University
  • Carolina Carrasco, Univ Autonoma Madrid, Dept Fis Mat Condensada
  • Pedro Jose de Pablo, Univ Autonoma Madrid, Dept Fis Mat Condensada
  • Julio Gomez-Herrero, Univ Autonoma Madrid, Dept Fis Mat Condensada
  • Arvind Raman, Birck Nanotechnology Center, Purdue University
Abstract

Dynamic atomic force microscopy is widely used for the imaging of soft biological materials in liquid environments; yet very little is known about the peak forces exerted by the oscillating probe tapping on the sample in liquid environments. In this article, we combine theory and experiments in liquid on virus capsids to propose scaling laws for peak interaction forces exerted on soft samples in liquid environments. We demonstrate how these laws can be used to choose probes and operating conditions to minimize imaging forces and thereby robustly image fragile biological samples.

Date of this Version
9-1-2008
Citation
DOI: 10.1529/biophysj.108.132829
Citation Information
Xin Xu, Carolina Carrasco, Pedro Jose de Pablo, Julio Gomez-Herrero, et al.. "Unmasking imaging forces on soft biological samples in liquids when using dynamic atomic force microscopy: A case study on viral capsids" (2008)
Available at: http://works.bepress.com/xinxu/1/