A three-step synthesis of a lipid-polymer conjugate is presented. The lipid, 1,2-di-O-hexadecyl-rac-glycerol (DHG) is conjugated to polyethylene glycol 2000 (PEG2000) via a pH-sensitive hydrazone linker. The steps involved in the synthesis of the conjugate include 1) incorporation of an ester group on the DHG moiety 2) hydrozinolysis of the ester group to yield a DHG hydrazide 3) condensation of the DHG hydrazide with m-PEG2000 aldehyde to yield the DHG-Hydrazone-PEG2000 conjugate. This hydrazone-based conjugate shows a pH-dependent hydrolysis and can be incorporated into liposomes to trigger cargo release at low pH environments as in cancer tissues.
Available at: http://works.bepress.com/xin-guo/51/