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Article
Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis
Biotechnology Letters
  • Xunlong Shi, Fudan University
  • Meiqing Feng, Fudan University
  • Yujie Zhao, Fudan University
  • Xin Guo, University of the Pacific
  • Pei Zhou, Fudan University
Document Type
Article
DOI
10.1007/s10529-007-9510-7
Publication Date
1-1-2008
Abstract
A recombinant Bacillus subtilis strain (KN25) was generated for the large-scale preparation of catalase. The B. subtilis katA gene encoding for catalase was cloned into the shuttle vector PRB374, downstream of the constitutively active vegII promoter, followed by transformation of the B. subtilis strain WB600 with the plasmid. The transformant strain, KN25 secretes high levels (3,500 U/ml) of catalase, which facilitates its purification. Three simple purification steps yielded nearly homogeneous catalase, with approximately 70% recovery. The purified recombinant catalase has a specific activity of 34,600 U/mg under optimal conditions, and is more resistant to acidic conditions than bovine liver catalase.
Citation Information
Xunlong Shi, Meiqing Feng, Yujie Zhao, Xin Guo, et al.. "Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis" Biotechnology Letters Vol. 30 Iss. 1 (2008) p. 181 - 186 ISSN: 0141-5492
Available at: http://works.bepress.com/xin-guo/27/