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Article
BCCIP regulates homologous recombination by distinct domains and suppresses spontaneous DNA damage
Nucleic acids research
  • Huimei Lu
  • Jingyin Yue
  • Xiangbing Meng, University of Iowa
  • Jac A. Nickoloff
  • Zhiyuan Shen
Document Type
Article
Peer Reviewed
1
Publication Date
12-1-2007
NLM Title Abbreviation
Nucleic Acids Res
DOI of Published Version
10.1093/nar/gkm732
Abstract

Homologous recombination (HR) is critical for maintaining genome stability through precise repair of DNA double-strand breaks (DSBs) and restarting stalled or collapsed DNA replication forks. HR is regulated by many proteins through distinct mechanisms. Some proteins have direct enzymatic roles in HR reactions, while others act as accessory factors that regulate HR enzymatic activity or coordinate HR with other cellular processes such as the cell cycle. The breast cancer susceptibility gene BRCA2 encodes a critical accessory protein that interacts with the RAD51 recombinase and this interaction fluctuates during the cell cycle. We previously showed that a BRCA2- and p21-interacting protein, BCCIP, regulates BRCA2 and RAD51 nuclear focus formation, DSB-induced HR and cell cycle progression. However, it has not been clear whether BCCIP acts exclusively through BRCA2 to regulate HR and whether BCCIP also regulates the alternative DSB repair pathway, non-homologous end joining. In this study, we found that BCCIP fragments that interact with BRCA2 or with p21 each inhibit DSB repair by HR. We further show that transient down-regulation of BCCIP in human cells does not affect non-specific integration of transfected DNA, but significantly inhibits homology-directed gene targeting. Furthermore, human HT1080 cells with constitutive down-regulation of BCCIP display increased levels of spontaneous single-stranded DNA (ssDNA) and DSBs. These data indicate that multiple BCCIP domains are important for HR regulation, that BCCIP is unlikely to regulate non-homologous end joining, and that BCCIP plays a critical role in resolving spontaneous DNA damage.

Published Article/Book Citation
Nucleic acids research, 35:21 (2007) pp.7160-7170.
Citation Information
Huimei Lu, Jingyin Yue, Xiangbing Meng, Jac A. Nickoloff, et al.. "BCCIP regulates homologous recombination by distinct domains and suppresses spontaneous DNA damage" Nucleic acids research Vol. 35 Iss. 21 (2007) p. 7160 - 7170 ISSN: 0305-1048
Available at: http://works.bepress.com/xiangbing_meng/5/