Modulation of the toxic effects of 2-aminoanthracene (2AA) on pancreatic tissue by apoptosis will be investigated. 2AA, also called anthramine, is a polycyclic aromatic hydrocarbon that is used in the manufacturing of chemicals, dyes, and inks, and it is also found in tobacco smoke and cooked foods. It is known to cause gene dysregulation, particularly of several genes of the pancreas that mediate protein and lipid metabolism (Gato and Means, 2011). To help determine the role of apoptosis in modulating the effects of 2AA, pancreatic tissue of Sprague Dawley rat dams exposed to various concentrations of 2AA for during gestation through postpartum will be analyzed for apoptotic activity using TUNEL apoptosis assay. This will be followed by total RNA extraction. The activity of Casp3, which plays a central role in the execution phase of apoptosis, will also be analyzed via assay, and relative gene expression of specific apoptotic genes will be quantified using qRT-PCR to test for significant differences in gene expression.
Available at: http://works.bepress.com/worlanyo_gato/70/