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Presentation
Role of Apoptosis in Modulating Effects of 2-Aminoanthracene in Pancreatic Tissue of Sprague Dawley Rat Dams
American Chemical Society National Meeting and Exposition (ACS)
  • Jacob W. Jay, Georgia Southern University
  • Emma Venable, Georgia Southern University
  • Elizabeth W. Howerth, University of Georgia
  • Worlanyo E. Gato, Georgia Southern University
Document Type
Presentation
Publication Date
3-22-2015
Disciplines
Abstract

Modulation of the toxic effects of 2-aminoanthracene (2AA) on pancreatic tissue by apoptosis will be investigated. 2AA, also called anthramine, is a polycyclic aromatic hydrocarbon that is used in the manufacturing of chemicals, dyes, and inks, and it is also found in tobacco smoke and cooked foods. It is known to cause gene dysregulation, particularly of several genes of the pancreas that mediate protein and lipid metabolism (Gato and Means, 2011). To help determine the role of apoptosis in modulating the effects of 2AA, pancreatic tissue of Sprague Dawley rat dams exposed to various concentrations of 2AA for during gestation through postpartum will be analyzed for apoptotic activity using TUNEL apoptosis assay. This will be followed by total RNA extraction. The activity of Casp3, which plays a central role in the execution phase of apoptosis, will also be analyzed via assay, and relative gene expression of specific apoptotic genes will be quantified using qRT-PCR to test for significant differences in gene expression.

Location
Denver, CO
Citation Information
Jacob W. Jay, Emma Venable, Elizabeth W. Howerth and Worlanyo E. Gato. "Role of Apoptosis in Modulating Effects of 2-Aminoanthracene in Pancreatic Tissue of Sprague Dawley Rat Dams" American Chemical Society National Meeting and Exposition (ACS) (2015)
Available at: http://works.bepress.com/worlanyo_gato/70/