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Presentation
The Role of Apoptosis in Modulating Effects of 2-Aminoanthracene in Fisher-344 Rat Liver
Environmental Mutagenesis and Genomics Society Annual Meeting (EMGS) (2013)
  • Worlanyo E. Gato, Georgia Southern University
  • Stacey McGee, Southern Illinois University
  • Dale B. Hales, Southern Illinois University
  • Jay C. Means, Southern Illinois University
Abstract
The arylamine 2-amino-anthracene (2-AA) is an aromatic hydrocarbon employed in manufacturing of chemicals, dyes, inks, and as curing agents in epoxy resins and polyurethanes. 2-AA has been detected tobacco smoke and cooked foods. The modulation of the toxic effects of 2-AA on the liver by apoptosis was investigated on 24 post weaning three-four week old F-344 male rats exposed to 0mg/kg-diet (control), 50mg/kg-diet (LD), 75mg/kg-diet (MD) and 100mg/kg-diet (HD) 2-AA for 14 [2WK] and 28 days [4WK]. Analysis of total mRNA extracts from liver for apoptosis-related gene expression changes in AEN, BAX, CASP3, JUN, MDM2, P53, and GAPDH genes by qRT-PCR was coupled with liver tissue histology (H&E staining), TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end-labeling) assay and Caspase (Caspase Glo assay) activity. Dose-related histological changes in liver cell architecture were observed at the highest doses in both 2wk and 4wk exposures. Dose-related increases in TUNEL-positive staining were also observed. Caspase3 assays showed dose-dependent increases (2WK) but suppression in LD and MD [4WK] rat livers and an increase in HD rats. Dose-related increases in expression were observed in all genes measured.
Keywords
  • Apoptosis,
  • Modulating effects,
  • 2-Aminoanthracene,
  • Fisher 344,
  • Rat liver
Disciplines
Publication Date
September 21, 2013
Location
Monterey, CA
Citation Information
Worlanyo E. Gato, Stacey McGee, Dale B. Hales and Jay C. Means. "The Role of Apoptosis in Modulating Effects of 2-Aminoanthracene in Fisher-344 Rat Liver" Environmental Mutagenesis and Genomics Society Annual Meeting (EMGS) (2013)
Available at: http://works.bepress.com/worlanyo_gato/11/