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Detection and quantification of Leptographium wageneri, the cause of black-stain root disease, from bark beetles (Coleoptera: Scolytidae) in Northern California using regular and Real-time PCR
Canadian Journal of Forest Research
  • Wolfgang Schweigkofler, Department of Environmental Science, Policy and Management, University of California, Berkeley
  • William J. Otrosina, USDA Forest Service, Southern Research Station
  • Sheri L. Smisth, USDA Forest Service, Pacific Southwest Region
  • Daniel R. Cluck, USDA Forest Service, Pacific Southwest Region
  • Kevin Maeda, Department of Environmental Science, Policy and Management, University of California, Berkeley
  • Kabir G. Peay, Department of Environmental Science, Policy and Management, University of California, Berkeley
  • Matteo Garbelotto, Department of Environmental Science, Policy and Management, University of California, Berkeley
Document Type
Article
Publication Date
8-1-2005
Department
Natural Sciences and Mathematics
Abstract
Black-stain root disease is a threat to conifer forests in western North America. The disease is caused by the ophiostomatoid fungus Leptographium wageneri (W.B. Kendr.) M.J. Wingf., which is associated with a number of bark beetle (Coleoptera: Scolytidae) and weevil species (Coleoptera: Curculionidae). We developed a polymerase chain reaction test to identify and quantify fungal DNA directly from insects. Leptographium wageneri DNA was detected on 142 of 384 bark beetle samples (37%) collected in Lassen National Forest, in northeastern California, during the years 2001 and 2002. Hylastes macer (LeConte) was the bark beetle species from which Leptographium DNA was amplified most regularly (2001: 63.4%, 2002: 75.0% of samples) . Lower insect?fungus association rates were found for Hylurgops porosus (LeConte), Hylurgops subcostulatus (Mannerheim), Hylastes gracilis (LeConte), Hylastes longicollis (Swaine), Dendroctonus valens (LeConte), and Ips pini (Say). The spore load per beetle ranged from 0 to over 1 × 105 spores, with only a few beetles carrying more than 1 × 103 spores. The technique permits the processing of a large number of samples synchronously, as required for epidemiological studies, to study infection rates in bark beetle populations and to identify potential insect vectors.
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Copyright © NRC Canada. All rights reserved.

Publisher Statement
Originally published as Schweigkofler, W., Otrosina, W. J., Smith, S. L., Cluck, D. R., Maeda, K., Peay, K. G., & Garbelotto, M. (2005). Detection and quantification of Leptographium wageneri, the cause of black-stain root disease, from bark beetles (Coleoptera: Scolytidae) in Northern California using regular and real-time PCR. Canadian Journal of Forest Research, 35(8), 1798-1808.
Citation Information
Wolfgang Schweigkofler, William J. Otrosina, Sheri L. Smisth, Daniel R. Cluck, et al.. "Detection and quantification of Leptographium wageneri, the cause of black-stain root disease, from bark beetles (Coleoptera: Scolytidae) in Northern California using regular and Real-time PCR" Canadian Journal of Forest Research Vol. 35 Iss. 8 (2005) p. 1798 - 1808 ISSN: 0045-5067
Available at: http://works.bepress.com/wolfgang_schweigkofler/32/