Article
Taxonomic Comparison of Different Thermophilic Sugar Beet Isolates with Glycosylated Surface Layer (S-Layer) Proteins and their Affiliation to Bacillus smithii
Systematic and Applied Microbiology
(1997)
Abstract
During the beet sugar campaign 1991/1992 we have characterized thermophilic bacteria from the extraction plant of an Austrian beet sugar factory in a polyphasic approach. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and freeze-fracture electron microscopy revealed the presence of weakly glycosylated, oblique crystalline cell surface layers (S-layers) on all sugar factory isolates. By partial 16S rDNA sequencing and DNA-DNA hybridisation it was demonstrated that the strains belong to the species Bacillus smithii. In the course of the campaign, however, different strains of B. smithii became dominant, which was demonstrated by fingerprinting using SDS-PAGE, polar lipid analysis, random amplified polymorphic DNA (RAPD) assays, fluorophore-assisted carbohydrate electrophoresis (FACE). Possible explanations for this divergence in strain development are discussed from the technological point of view.
Keywords
- Bacillus smithii,
- surface layer (S-layer),
- prokaryotic glycoprotein,
- taxonomy,
- polar lipids,
- RAPD analysis,
- 16S rDNA sequencing,
- DNA-DNA hybridisation
Disciplines
Publication Date
November, 1997
Citation Information
Paul Messner, Andrea Scheberl, Wolfgang Schweigkofler, Friedrich Hollaus, et al.. "Taxonomic Comparison of Different Thermophilic Sugar Beet Isolates with Glycosylated Surface Layer (S-Layer) Proteins and their Affiliation to Bacillus smithii" Systematic and Applied Microbiology Vol. 20 Iss. 4 (1997) p. 559 - 565 ISSN: 0723-2020 Available at: http://works.bepress.com/wolfgang_schweigkofler/23/