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Diagnosis of Aeromonas hydrophila, Mycobacterium species, and Batrachochytrium dendrobatidis in an African clawed frog (Xenopus laevis).
Journal of the American Association for Laboratory Animal Science : JAALAS (2010)
  • William Allen Hill, University of Tennessee, Knoxville
  • Shelley J. Newman, University of Tennessee - Knoxville
  • Linden Craig, University of Tennessee
  • Chris Carter, University of Tennessee - Knoxville
  • Jane Czarra, University of Tennessee - Knoxville
  • Julie Paige Brown, University of Tennessee - Knoxville
Abstract
Here we describe diagnosis of concurrent infection with Aeromonas hydrophila, Mycobacterium spp., and Batrachochytrium dendrobatidis in a wild female Xenopus laevis captured in Chile and transported to the United States. After approximately 130 d in the laboratory, the frog was presented for dysecdysis and obtundation. After euthanasia, tissues were submitted for histopathologic evaluation and PCR analysis for B. dendrobatidis and Ranavirus. Clinically significant gross lesions included cutaneous ulcerations on the lip, right forelimb, and ventral chest. Microscopic findings included regionally extensive splenic necrosis, diffuse pneumonia, and fibrinous coelomitis all containing intralesional bacteria. PCR analysis yielded positive results for B. dendrobatidis only. Bacterial culture of the ulcerated skin and liver yielded A. hydrophila. Infection with Contracaecum spp. was diagnosed as an incidental finding. To our knowledge, this case is the first report of simultaneous infection with Aeromonas hydrophila, Mycobacterium spp., and Batrachochytrium dendrobatidis in a laboratory-maintained X. laevis captured from the wild.
Publication Date
March, 2010
Citation Information
William Allen Hill, Shelley J. Newman, Linden Craig, Chris Carter, et al.. "Diagnosis of Aeromonas hydrophila, Mycobacterium species, and Batrachochytrium dendrobatidis in an African clawed frog (Xenopus laevis)." Journal of the American Association for Laboratory Animal Science : JAALAS Vol. 49 Iss. 2 (2010)
Available at: http://works.bepress.com/william_hill/8/