Skip to main content
Article
Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection based on DNA Origami Nanostructures
ACS Omega
  • Shuo Han
  • Wenyan Liu, Missouri University of Science and Technology
  • Shuo Yang
  • Risheng Wang, Missouri University of Science and Technology
Abstract

MicroRNAs (miRNAs) have emerged as the promising molecular biomarkers for early diagnosis and enhanced understanding of the molecular pathogenesis of cancers as well as certain diseases. Here, a facile, label-free, and amplification-free electrochemical biosensor was developed to detect miRNA by using DNA origami nanostructure-supported DNA probes, with methylene blue (MB) serving as the hybridization redox indicator, for the first time. Specifically, the use of cross-shaped DNA origami nanostructures containing multiple single-stranded DNA probes at preselected locations on each DNA nanostructure could increase the accessibility and the recognition efficiency of the probes (due to the rational controlled density of DNA probes). The successful immobilization of DNA origami probes and their hybridization with targeted miRNA-21 molecules was confirmed by electrochemical impedance spectroscopy and cyclic voltammetry methods. A differential pulse voltammetry technique was employed to record the oxidation peak current of MB before and after target hybridization. The linear detection range of this biosensor was from 0.1 pM to 10.0 nM, with a lower detection limit of 79.8 fM. The selectivity of the miRNA biosensor was also studied by observing the discrimination ability of single-base mismatched sequences. Because of the larger surface area and unprecedented customizability of DNA origami nanostructures, this strategy demonstrated great potential for sensitive, selective, and label-free determination of miRNA for translational biomedical research and clinical applications.

Department(s)
Chemistry
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2019 American Chemical Society (ACS), All rights reserved.
Publication Date
6-1-2019
Disciplines
Citation Information
Shuo Han, Wenyan Liu, Shuo Yang and Risheng Wang. "Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection based on DNA Origami Nanostructures" ACS Omega Vol. 4 Iss. 6 (2019) p. 11025 - 11031 ISSN: 2470-1343
Available at: http://works.bepress.com/wenyan-liu/16/