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Article
A DNA vaccine prime followed by a liposome-encapsulated protein boost confers enhanced mucosal immune responses and protection
GSBS Student Publications
  • Kejian Yang
  • Barbara J. Whalen
  • Rebecca S Tirabassi
  • Liisa K. Selin, University of Massachusetts Medical School
  • Tatyana S. Levchenko
  • Vladimir P. Torchilin
  • Edward H. Kislauskis, University of Massachusetts Medical School
  • Dennis L. Guberski
UMMS Affiliation
Graduate School of Biomedical Sciences; Oral Vaccine Institute; Department of Pathology
Date
4-22-2008
Document Type
Article
Medical Subject Headings
Administration, Intranasal; Animals; Animals, Newborn; Antibody Formation; Female; Hepatitis B Surface Antigens; Hepatitis B Vaccines; Humans; Immunity, Mucosal; *Immunization, Secondary; Liposomes; Mice; Mice, Inbred BALB C; Th1 Cells; Vaccines, DNA; Vaccinia virus
Abstract

A variety of DNA vaccine prime and recombinant viral boost immunization strategies have been developed to enhance immune responses in humans, but inherent limitations to these strategies exist. There is still an overwhelming need to develop safe and effective approaches that raise broad humoral and T cell-mediated immune responses systemically and on mucosal surfaces. We have developed a novel mucosal immunization regimen that precludes the use of viral vectors yet induces potent T cell responses. Using hepatitis B surface Ag (HBsAg), we observed that vaccination of BALB/c mice with an i.m. HBsAg-DNA vaccine prime followed by an intranasal boost with HBsAg protein encapsulated in biologically inert liposomes enhanced humoral and T cell immune responses, particularly on mucosal surfaces. Intranasal live virus challenge with a recombinant vaccinia virus expressing HBsAg revealed a correlation between T cell immune responses and protection of immunized mice. A shortened immunization protocol was developed that was successful in both adult and neonatal mice. These results support the conclusion that this new approach is capable of generating a Th-type-1-biased, broad spectrum immune response, specifically at mucosal surfaces. The success of this design may provide a safe and effective vaccination alternative for human use.

Rights and Permissions
Citation: J Immunol. 2008 May 1;180(9):6159-67.
Related Resources
Link to Article in PubMed
PubMed ID
18424737
Citation Information
Kejian Yang, Barbara J. Whalen, Rebecca S Tirabassi, Liisa K. Selin, et al.. "A DNA vaccine prime followed by a liposome-encapsulated protein boost confers enhanced mucosal immune responses and protection" Vol. 180 Iss. 9 (2008) ISSN: 0022-1767 (Print)
Available at: http://works.bepress.com/vtorchilin/1/