Article
A Novel Transgenic Line of Mice Exhibiting Autosomal Recessive Male-Specific Lethality and Non-Alcoholic Fatty Liver Disease
Biochemistry and Microbiology
Document Type
Article
Publication Date
10-1-2002
Disciplines
Abstract
We have isolated a Meis1a transgenic mouse line exhibiting recessive male-specific lethality and nonalcoholic fatty liver disease (NAFLD), which coincides with pubescence and is androgen-dependent. The phenotype is due to disruption of an endogenous locus, since other Meis1a transgenic lines do not exhibit these phenotypes. Necropsy analysis revealed hepatic microvesicular steatosis in pubescent male homozygous mice, which is absent in transgenic females. The transgene insertion site was localized to chromosome 1 and further refined by cloning the flanking regions. Sequence analysis shows that the integration site disrupts a putative metallo-b-lactamase gene with a 21.3 kb deletion encompassing exons 5–7.
Citation Information
Sollars VE, McEntee BJ, Engiles JB, Rothstein JL, Buchberg AM. A novel transgenic line of mice exhibiting autosomal recessive male-specific lethality and non-alcoholic fatty liver disease. Human Molecular Genetics 22: 2777–86, 2002.
The full text (HTML) and the published PDF versions of this article are available on the open Web. doi:10.1093/hmg/11.22.2777