Skip to main content
Enzyme-Amplified Array Sensing of Proteins in Solution and in Biofluids
Journal of the American Chemical Society (2010)
  • OR Miranda
  • HT Chen
  • CC You
  • DE Mortenson
  • XC Yang
  • UHF Bunz
  • VM Rotello

We have developed an enzyme−nanoparticle sensor array where the sensitivity is amplified through enzymatic catalysis. In this approach cationic gold nanoparticles are electrostatically bound to an enzyme (β-galactosidase, β-Gal), inhibiting enzyme activity. Analyte proteins release the β-Gal, restoring activity and providing an amplified readout of the binding event. Using this strategy we have been able to identify proteins in buffer at a concentration of 1 nM, substantially lower than current strategies for array-based protein sensing. Moreover, we have obtained identical sensitivity in studies where the proteins are spiked into the complex protein matrix provided by desalted human urine (1.5 μM total protein; spiked protein concentrations were 0.067% of the overall protein concentration), demonstrating the potential of the method for diagnostic applications.

Publication Date
March 23, 2010
Publisher Statement
DOI: 10.1021/ja1006756
Citation Information
OR Miranda, HT Chen, CC You, DE Mortenson, et al.. "Enzyme-Amplified Array Sensing of Proteins in Solution and in Biofluids" Journal of the American Chemical Society Vol. 132 Iss. 14 (2010)
Available at: