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The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Cell (1993)
  • Rosalind C. Lee, Harvard University
  • Rhonda L. Feinbaum, Harvard University
  • Victor R. Ambros, Harvard University
Abstract
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate the gene from three other Caenorhabditis species; all four Caenorhabditis clones functionally rescue the lin-4 null allele of C. elegans. Comparison of the lin-4 genomic sequence from these four species and site-directed mutagenesis of potential open reading frames indicated that lin-4 does not encode a protein. Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction.
Publication Date
December 3, 1993
Publisher Statement
Citation: Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 1993 Dec 3;75(5):843-54. PubMed PMID: 8252621.
Citation Information
Rosalind C. Lee, Rhonda L. Feinbaum and Victor R. Ambros. "The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14" Cell Vol. 75 Iss. 5 (1993)
Available at: http://works.bepress.com/victor_ambros/77/