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The Timing oflin-4RNA Accumulation Controls the Timing of Postembryonic Developmental Events inCaenorhabditis elegans
Developmental Biology (1999)
  • Rhonda Feinbaum, Massachusetts General Hospital
  • Victor R. Ambros, Dartmouth College
Abstract
The lin-4 gene encodes a small RNA that is required to translationally repress lin-14 toward the end of the first larval stage of Caenorhabditis elegans development. To determine if the timing of LIN-14 protein down-regulation depends on the temporal profile of lin-4 RNA level, we analyzed the stage-specificity of lin-4 RNA expression during wild-type development and examined the phenotypes of transgenic worms that overexpress lin-4 RNA during the first larval stage. We found that lin-4 RNA first becomes detectable at approximately 12 h of wild-type larval development and rapidly accumulates to nearly maximum levels by 16 h. This profile of lin-4 RNA accumulation corresponded to the timing of LIN-14 protein down-regulation. Transgenic strains that express elevated levels of lin-4 RNA prior to 12 h of development display reduced levels of LIN-14 protein and precocious phenotypes consistent with abnormally early loss of lin-14 activity. These results indicate that the temporal profile of lin-4 RNA accumulation specifies the timing of LIN-14 down-regulation and thereby controls the timing of postembryonic developmental events.
Publication Date
June 1, 1999
Citation Information
Rhonda Feinbaum and Victor R. Ambros. "The Timing oflin-4RNA Accumulation Controls the Timing of Postembryonic Developmental Events inCaenorhabditis elegans" Developmental Biology Vol. 210 Iss. 1 (1999)
Available at: http://works.bepress.com/victor_ambros/67/