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The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis in Drosophila melanogaster
Developmental Biology (2002)
  • Lorenzo F. Sempere
  • Edward B. Dubrovsky, Dartmouth College
  • Veronica A. Dubrovskaya, Dartmouth College
  • Edward M. Berger, Dartmouth College
  • Victor R. Ambros
Abstract
In Caenorhabditis elegans, the heterochronic pathway controls the timing of developmental events during the larval stages. A component of this pathway, the let-7 small regulatory RNA, is expressed at the late stages of development and promotes the transition from larval to adult (L/A) stages. The stage-specificity of let-7 expression, which is crucial for the proper timing of the worm L/A transition, is conserved in Drosophila melanogaster and other invertebrates. In Drosophila, pulses of the steroid hormone 20-hydroxyecdysone (ecdysone) control the timing of the transition from larval to pupal to adult stages. To test whether let-7 expression is regulated by ecdysone in Drosophila, we used Northern blot analysis to examine the effect of altered ecdysone levels on let-7 expression in mutant animals, organ cultures, and S2 cultured cells. Experiments were conducted to test the role of Broad-Complex (BR-C), an essential component in the ecdysone pathway, in let-7 expression. We show that ecdysone and BR-C are required for let-7 expression, indicating that the ecdysone pathway regulates the temporal expression of let-7 in Drosophila. These results demonstrate an interaction between steroid hormone signaling and the heterochronic pathway in insects.
Publication Date
April 1, 2002
Citation Information
Lorenzo F. Sempere, Edward B. Dubrovsky, Veronica A. Dubrovskaya, Edward M. Berger, et al.. "The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis in Drosophila melanogaster" Developmental Biology Vol. 244 Iss. 1 (2002)
Available at: http://works.bepress.com/victor_ambros/58/