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Biodistribution and function of extracellular miRNA-155 in mice
Program in Molecular Medicine Publications and Presentations
  • Shashi Bala, University of Massachusetts Medical School
  • Timea Csak, University of Massachusetts Medical School
  • Fatemeh Momen-Heravi, University of Massachusetts Medical School
  • Dora Lippai, University of Massachusetts Medical School
  • Karen Kodys, University of Massachusetts Medical School
  • Donna Catalano, University of Massachusetts Medical School
  • Abhishek Satishchandran, University of Massachusetts Medical School
  • Victor R. Ambros, University of Massachusetts Medical School
  • Gyongyi Szabo, University of Massachusetts Medical School
UMMS Affiliation
Department of Medicine, Division of Gastroenterology; Program in Molecular Medicine
Date
5-29-2015
Document Type
Article
Medical Subject Headings
MicroRNAs
Abstract
Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. Administration of exosomes loaded with synthetic miR-155 mimic into miR-155 KO mice resulted in a rapid accumulation and clearance of miR-155 in the plasma with subsequent distribution in the liver, adipose tissue, lung, muscle and kidney (highest to lowest, respectively). miR-155 expression was detected in isolated hepatocytes and liver mononuclear cells of recipient KO mice suggesting its cellular uptake. In vitro, exosome-mediated restoration of miR-155 in Kupffer cells from miR-155 deficient mice augmented their LPS-induced MCP1 mRNA increase. The systemic delivery of wild type plasma to miR-155 KO mice also resulted in a rapid accumulation of miR-155 in the circulation and distribution to the liver and adipose tissue. In summary, our results demonstrate tissue biodistribution and biologic function of EV-associated miR-155.
Comments

Citation: Sci Rep. 2015 May 29;5:10721. doi: 10.1038/srep10721. Link to article on publisher's site

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Related Resources
Link to Article in PubMed
PubMed ID
26024046
Creative Commons License
Creative Commons Attribution 4.0
Citation Information
Shashi Bala, Timea Csak, Fatemeh Momen-Heravi, Dora Lippai, et al.. "Biodistribution and function of extracellular miRNA-155 in mice" Vol. 5 (2015) ISSN: 2045-2322 (Linking)
Available at: http://works.bepress.com/victor_ambros/48/