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Article
Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA promoter activity
Open Access Articles
  • Natalia Julia Martinez, University of Massachusetts Medical School
  • Maria C. Ow, University of Massachusetts Medical School
  • John S. Reece-Hoyes, University of Massachusetts Medical School
  • M. Inmaculada Barrasa, University of Massachusetts Medical School
  • Victor R. Ambros, University of Massachusetts Medical School
  • Albertha J. M. Walhout, University of Massachusetts Medical School
UMMS Affiliation
Program in Gene Function and Expression
Date
11-5-2008
Document Type
Article
Subjects
Animals; Animals, Genetically Modified; Blotting, Northern; Caenorhabditis elegans; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; *Genes, Helminth; *Genome, Helminth; Green Fluorescent Proteins; *MicroRNAs; *Promoter Regions, Genetic; Protein Processing, Post-Translational; RNA, Helminth; Time Factors
Abstract
The Caenorhabditis elegans genome encodes more than 100 microRNAs (miRNAs). Genetic analyses of miRNA deletion mutants have only provided limited insights into miRNA function. To gain insight into the function of miRNAs, it is important to determine their spatiotemporal expression pattern. Here, we use miRNA promoters driving the expression of GFP as a proxy for miRNA expression. We describe a set of 73 transgenic C. elegans strains, each expressing GFP under the control of a miRNA promoter. Together, these promoters control the expression of 89 miRNAs (66% of all predicted miRNAs). We find that miRNA promoters drive GFP expression in a variety of tissues and that, overall, their activity is similar to that of protein-coding gene promoters. However, miRNAs are expressed later in development, which is consistent with functions after initial body-plan specification. We find that miRNA members belonging to families are more likely to be expressed in overlapping tissues than miRNAs that do not belong to the same family, and provide evidence that intronic miRNAs may be controlled by their own, rather than a host gene promoter. Finally, our data suggest that post-transcriptional mechanisms contribute to differential miRNA expression. The data and strains described here will provide a valuable guide and resource for the functional analysis of C. elegans miRNAs.
Rights and Permissions
Citation: Genome Res. 2008 Dec;18(12):2005-15. Epub 2008 Nov 3. Link to article on publisher's site
DOI of Published Version
10.1101/gr.083055.108
Related Resources
Link to Article in PubMed
PubMed ID
18981266
Citation Information
Natalia Julia Martinez, Maria C. Ow, John S. Reece-Hoyes, M. Inmaculada Barrasa, et al.. "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA promoter activity" Vol. 18 Iss. 12 (2008) ISSN: 1088-9051 (Print)
Available at: http://works.bepress.com/victor_ambros/34/