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Article
Systematic analysis of dynamic miRNA-target interactions during C. elegans development
Program in Molecular Medicine Publications and Presentations
  • Liang Zhang, University of Colorado, Boulder
  • Molly Hammell, University of Massachusetts Medical School
  • Brian A. Kudlow, University of Colorado, Boulder
  • Victor R. Ambros, University of Massachusetts Medical School
  • Min Han, University of Colorado, Boulder
UMMS Affiliation
Program in Molecular Medicine
Date
8-14-2009
Document Type
Article
Medical Subject Headings
3' Untranslated Regions; Animals; *Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; Gene Expression Profiling; *Gene Expression Regulation, Developmental; MicroRNAs; Oligonucleotide Array Sequence Analysis; RNA, Messenger; Recombinant Fusion Proteins
Disciplines
Abstract

Although microRNA (miRNA)-mediated functions have been implicated in many aspects of animal development, the majority of miRNA::mRNA regulatory interactions remain to be characterized experimentally. We used an AIN/GW182 protein immunoprecipitation approach to systematically analyze miRNA::mRNA interactions during C. elegans development. We characterized the composition of miRNAs in functional miRNA-induced silencing complexes (miRISCs) at each developmental stage and identified three sets of miRNAs with distinct stage-specificity of function. We then identified thousands of miRNA targets in each developmental stage, including a significant portion that is subject to differential miRNA regulation during development. By identifying thousands of miRNA family-mRNA pairs with temporally correlated patterns of AIN-2 association, we gained valuable information on the principles of physiological miRNA::target recognition and predicted 1589 high-confidence miRNA family::mRNA interactions. Our data support the idea that miRNAs preferentially target genes involved in signaling processes and avoid genes with housekeeping functions, and that miRNAs orchestrate temporal developmental programs by coordinately targeting or avoiding genes involved in particular biological functions.

Rights and Permissions
Citation: Development. 2009 Sep;136(18):3043-55. Epub 2009 Aug 12. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
19675127
Citation Information
Liang Zhang, Molly Hammell, Brian A. Kudlow, Victor R. Ambros, et al.. "Systematic analysis of dynamic miRNA-target interactions during C. elegans development" Vol. 136 Iss. 18 (2009) ISSN: 0950-1991 (Linking)
Available at: http://works.bepress.com/victor_ambros/13/