- Stem cells,
- Gene expression,
- Ligaments,
- Immune System,
- Sociology,
- Statistics,
- Media,
- Patient Treatment,
- Biochemistry,
- Cellular Biophysics,
- Genetics,
- Neurophysiology,
- time 6 day,
- pluripotent adult stem cell source,
- periodontal ligament stem cells,
- regenerative medicine,
- in-vivo germ layers,
- in-vitro neurogenesis,
- neuroinduced Connexin 43+ PDLSC,
- neurogenic cocktail media,
- cell morphology,
- gene expression,
- immunohistochemistry,
- two-tailed t-test,
- neuro filament medium,
- neural lineage
A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<;0.05 considered significant. Morphological differences were evident in neuro-induced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuro-induced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.
Available at: http://works.bepress.com/veronica-fortino/12/