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Apolipoprotein E Pathology in Vascular Dementia
International Journal of Clinical and Experimental Pathology
  • Troy T. Rohn, Boise State University
  • Ryan J. Day, Boise State University
  • Colin B. Sheffield, Boise State University
  • Alexander J. Rajic, University of California - Irvine
  • Wayne W. Poon, University of California - Irvine
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Vascular dementia (VaD) is the second most common form of dementia and is currently defined as a cerebral vessel vascular disease leading to ischemic episodes. Apolipoprotein E (apoE) gene polymorphism has been proposed as a risk factor for VaD, however, to date there are few documented post-mortem studies on apoE pathology in the VaD brain. To investigate a potential role for the apoE protein, we analyzed seven confirmed cases of VaD by immunohistochemistry utilizing an antibody that specifically detects the amino-terminal fragment of apoE. Application of this antibody, termed N-terminal, apoE cleavage fragment (nApoECF) revealed consistent labeling within neurofibrillary tangles (NFTs), blood vessels, and reactive astrocytes. Labeling occurred in VaD cases that had confirmed APOE genotypes of 3/3, 3/4, and 4/4, with respect to NFTs, staining of the nApoECF co-localized with PHF-1 and was predominantly localized to large, stellate neurons in layer II of the entorhinal cortex. Quantitative analysis indicated that approximately 38.4% of all identified NFTs contained the amino-terminal fragment of apoE. Collectively, these data support a role for the proteolytic cleavage of apoE in the VaD and support previous reports that APOE polymorphism is significantly associated with susceptibility in this disease.
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This document was originally published by e-Century Publishing in International Journal of Clinical and Experimental Pathology. This work is provided under a Creative Commons Attribution-NonCommercial 4.0 License. Details regarding the use of this work can be found at:

Citation Information
Troy T. Rohn, Ryan J. Day, Colin B. Sheffield, Alexander J. Rajic, et al.. "Apolipoprotein E Pathology in Vascular Dementia" International Journal of Clinical and Experimental Pathology (2014)
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