In 1961, Koch demonstrated successful treatment of cancer and other chronic diseases by injecting low concentrations of a carbonyl compound of type R(CO.CO)nR′. The data indicate that at most two molecules per cancer cell are bound and destroy the cell. We model the process as single molecules binding within sub-compartments of cancer cells following migration through diffusion channels opened up by the disease. We assume that the volume of the sub-compartment binding a single molecule is so small that binding occurs much faster than diffusion. Inside the sub-compartment binding occurs in one of two modes: either single binding with subsequent isomerization or an allosteric switch, with either mode leading to cell death. We study the use of micro-fluorimetry with 2-photon absorption to locate the binding site of the molecule and to distinguish between the two alternative binding modes. Equations describing the uptake of the molecules by cancer cells in the human body and the binding of these molecules within sub-compartments are presented, and the kinetics are numerically simulated. These simulations facilitate the design of clinical studies and the development of an experimental design to distinguish between the two possible binding modes of single molecules in submicron compartments.
- Allosterism in membranes,
- Low dissociation constants
Available at: http://works.bepress.com/tjalling_ypma/2/