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Article
Flow rate and duty cycle effects in lysis of Chlamydomonas reinhardtii using high-energy pulsed focused ultrasound
Journal of the Acoustical Society of America
  • Grant Riesberg, Iowa State University
  • Timothy A. Bigelow, Iowa State University
  • Dan Joseph Stessman, Iowa State University
  • Martin H. Spalding, II, Iowa State University
  • Linxing Yao, Iowa State University
  • Tong Wang, Iowa State University
  • Jin Xu, John Brown University
Document Type
Article
Publication Version
Published Version
Publication Date
6-1-2014
DOI
10.1121/1.4874627
Abstract
To consider microalgae lipid biofuel as a viable energy source, it is a necessity to maximize algal cell lysis, lipid harvest, and thus biofuel production versus the energy used to lyse the cells. Previous techniques have been to use energy consumptive ultrasound waves in the 10-40 kHz range in a stationary exposure environment. This study evaluated the potential of using 1.1 MHz ultrasound pulses in a new flow through type chamber on Chlamydomonas reinhardtii as a model organism for cell breakage. The ultrasound was generated using a spherically focused transducer with a focal length of 6.34 cm and an active diameter of 6.36 cm driven by 20 cycle sine-wave tone bursts at varied pulse repetition frequencies. First, variations in flow rate were examined at a constant duty cycle of 3.6%. After assessing flow rates, the duty cycle was varied to further explore the dependence on the tone burst parameters. Cell lysis was assessed by quantifying protein and chlorophyll release into the supernatant as well as by lipid extractability. Appropriate flow rates with higher duty cycles led to statistically significant increases in cell lysis relative to controls and other exposure conditions.
Comments

The following article appeared in Journal of the Acoustical Society of America 135 (2014): 3632 and may be found at doi: 10.1121/1.4874627.

Rights
Copyright 2014 Acoustical Society of America. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the Acoustical Society of America.
Copyright Owner
Acoustical Society of America
Language
en
File Format
application/pdf
Citation Information
Grant Riesberg, Timothy A. Bigelow, Dan Joseph Stessman, Martin H. Spalding, et al.. "Flow rate and duty cycle effects in lysis of Chlamydomonas reinhardtii using high-energy pulsed focused ultrasound" Journal of the Acoustical Society of America Vol. 135 Iss. 6 (2014) p. 3632 - 3638
Available at: http://works.bepress.com/timothy_bigelow/17/