Rates of depression, anxiety, and posttraumatic stress disorder (PTSD) are greater in youth living with HIV (YLWH) than in the general population. Currently, depression screening guidelines exist for YLWH but not anxiety and PTSD. This study examined data from comprehensive mental health screenings conducted with YLWH, which included measures of depression, anxiety, and PTSD. Percentages of positive screeners were 35%, 25.7%, and 20.6% for depression, anxiety, and PTSD, respectively. Older participants reported more depression and anxiety symptoms. Participants with less education reported more PTSD symptoms. These findings support the need for universal and comprehensive mental health screenings for YLWH.