Biologists doing high-throughput high-content cellular analysis are generally not computer scientists or high performance computing (HPC) experts, and they want their workflow to support their science without having to be. We describe a new HPC enabled data analytics workflow with a web interface, HPC pipeline for analysis, and both traditional and new analytics tools to help them transition from a single workstation mode of operation to power HPC users. This allows the processing of multiple plates over a short period of time to ensure timely query and analysis to match potential countermeasures to individual responses.